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Medical examination microbiology graduation thesis essay

编辑: 毕业论文 Release time: 2019-05-02 Editor: Graduation Thesis

Purpose: The disease-causing bacteria, as the most important inspection index in the medical diagnosis and meat product health inspection industry, has always been concerned by bacteria professionals worldwide. Finding this rapid and simple microbial detection process and its layout, an emergency response mechanism applicable to all meat processing processes, has become the first key way to manipulate the susceptibility of diseased bacteria and the environmental damage of meat products. Cholesterol and nucleic acids are the two most important types of bacterial ions in bacteria. With the help of antigens or trace nucleic acids, relying on different fluorescent initiators or sensor technologies has long become the frontier of exploration for the detection of disease-causing microorganisms in today's society. . This discussion is to design and plan a series of new rapid detection methods of disease-causing bacteria and their preventive application mechanisms based on their overview technology, in order to reduce the susceptibility of the disease-causing bacteria and environmental damage to meat products.


Methods: (1) Relying on the quenching characteristics of nano-gold particles for fluorescent dyes, and using nucleic acid aptamers to target specific ions with high non-specific affinity potential, according to single-stranded DNA nucleic acid aptamers of Streptococcus hemolyticus, both can interact with The specific fusion of the bacterial protein can also generate double-stranded properties with complementary oligonucleotides. After the specific sample is introduced to reflect the mechanism, the fluorescent signal changes from quenching to agitation, and the hemolytic property is maintained according to the intensity of the fluorescent signal. Inspection of streptococcus. (2) Relying on the distinctive ionic beacons and complementary nucleotide sequence layouts lacking DNA sites, proper use of DNA stem-loop construction and complementary nucleic acid sequence hybridization to complement each other to form specific DNA sequences of Streptococcus orange yellow, according to the characteristics of nucleosides The change of the acid structure and the configuration phase drove the ATMND (2-amino-5,6,7-trimethyl-1,8-naphthyridine) dye to leave the missing site and the fluorescence was excited again. According to the fluorescence signal intensity test, To distinguish the presence of Streptococcus orange. (3) Based on independent immunization of Escherichia coli 0157: H7 bacterial body antigen, creating hybridoma cell lines, and using blood cell agglutination test and ELISA to evaluate titer and other methods, independently immunize and obtain Escherichia coli 0157 : H7 monoclonal antibody. (4) The development of a non-specific enzyme-linked immunoassay for antigenic antibodies in this application scenario reflects the rapid detection of a photocatalytic bacteria-free immunity method for Escherichia coli 0157: H7. The method uses a dual-antibody sandwich enzyme-linked immunization reaction mechanism, integrates the characteristics of catalytic silver such as alkaline phosphatase, and uses tempered steel as a micro-void interstitial electric level array control device with integrated IC structure layer, according to the biosensor photocatalysis related signal intensity The transformation was maintained on the dissection of Escherichia coli 0157: H7. (5) Based on the analysis of bacterial damage and important control point mechanisms of food processing companies, carry out harmful interference to sauce and meat products companies that are likely to cause bacterial environmental damage, and then evaluate based on the control points of bacterial adverse effects to find important control of bacterial environmental damage in the production process At the point of the stage, we will create a mechanism for preventing bacterial environmental damage, and apply traditional bacterial culture methods and independent design and planning methods such as non-marker dye ion beacon electrode technology to comprehensively reduce bacterial environmental damage.

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